Coding

Part:BBa_K3629020:Design

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-12)


Yarrowia lipolytica TRP2 Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1294
    Illegal NgoMIV site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 283
    Illegal BsaI.rc site found at 544
    Illegal BsaI.rc site found at 1441


Design Notes

Below is the codon-optimized sequence of Yarrowia lipolytica TRP2 for use in any expression construct. However, we have also made our own expression construct BBa_K3629024, designed to allow for optimal expression of TRP2 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Source

From genome sequence of Yarrowia lipolytica CDS: T01033.

References

1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)